Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

Kumar, Adepu K; Parikh, Bhumika S; Singh, Surendra P; Shah, Deval

doi:10.1186/s40643-015-0062-8

Bioresources and Bioprocessing

Table 2 Enzyme activities from the wild-type and mutant strains grown on RS, BG and mixed RSBG growth substrates

From: Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

Strain	Growth substrate	Enzyme activity (U mL⁻¹)
Strain	Growth substrate	FPase	Avicelase	CMCase	β-Glucosidase	Xylanase
WT	Rice straw	0.61 ± 0.05	0.97 ± 0.03	15.31 ± 1.2	4.87 ± 0.25	267 ± 7.0
UV1		1.41 ± 0.09	3.72 ± 0.05	36.83 ± 1.7	8.50 ± 0.43	204 ± 6.7
UV2		1.34 ± 0.08	1.51 ± 0.03	38.16 ± 2.0	13.5 ± 0.62	235 ± 7.6
EMS1		1.67 ± 0.1	1.59 ± 0.05	35.47 ± 1.1	11.37 ± 0.56	242 ± 6.1
EMS2		2.07 ± 0.06	2.31 ± 0.03	33.11 ± 1.3	5.12 ± 0.25	266 ± 7.3
WT	Sugarcane bagasse	0.93 ± 0.05	0.47 ± 0.06	17.89 ± 0.9	4.68 ± 0.01	583 ± 7.7
UV1		1.27 ± 0.06	1.00 ± 0.11	35.84 ± 1.4	6.87 ± 0.56	552 ± 8.1
UV2		1.70 ± 0.05	0.42 ± 0.1	31.59 ± 1.2	8.12 ± 0.31	616 ± 8.8
EMS1		1.74 ± 0.07	0.95 ± 0.1	38.00 ± 1.6	9.43 ± 0.75	646 ± 10
EMS2		1.69 ± 0.06	0.95 ± 0.11	39.8 ± 1.7	9.12 ± 1.0	656 ± 9.8
WT	RS75:BG25	0.52 ± 0.03	0.50 ± 0.02	6.21 ± 0.6	4.0 ± 0.18	138 ± 6.2
UV1		1.56 ± 0.08	0.67 ± 0.11	28.95 ± 1.5	15.5 ± 0.43	215 ± 6.6
UV2		1.41 ± 0.05	0.20 ± 0.006	26.57 ± 1.3	16.0 ± 0.68	214 ± 6.9
EMS1		1.35 ± 0.06	0.18 ± 0.006	21.03 ± 1.1	12.5 ± 0.81	228 ± 7.5
EMS2		2.09 ± 0.11	0.30 ± 0.01	30.42 ± 1.3	19.9 ± 1.12	252 ± 6.5
WT	RS50:BG50	0.71 ± 0.04	0.45 ± 0.06	7.99 ± 0.8	4.56 ± 0.12	273 ± 7.8
UV1		1.46 ± 0.05	0.35 ± 0.06	33.53 ± 1.5	7.93 ± 0.56	260 ± 7.0
UV2		1.60 ± 0.03	0.16 ± 0.06	24.75 ± 1.2	10.5 ± 1.12	222 ± 6.4
EMS1		1.64 ± 0.02	0.34 ± 0.01	25.54 ± 1.1	10.0 ± 1.0	240 ± 7.2
EMS2		2.01 ± 0.07	0.48 ± 0.06	32.20 ± 1.4	12.37 ± 1.12	268 ± 6.3
WT	RS25:BG75	0.67 ± 0.02	0.30 ± 0.01	9.15 ± 0.8	3.93 ± 0.06	292 ± 6.8
UV1		1.45 ± 0.03	0.27 ± 0.01	27.19 ± 1.1	6.06 ± 0.25	185 ± 6.1
UV2		1.95 ± 0.05	0.52 ± 0.06	25.87 ± 1.3	7.93 ± 1.31	320 ± 7.5
EMS1		2.34 ± 0.06	0.67 ± 0.08	30.92 ± 1.2	7.43 ± 1.06	285 ± 8.2
EMS2		1.91 ± 0.05	0.62 ± 0.01	21.52 ± 1.1	7.25 ± 0.81	324 ± 8.0

Values are the average of three repetitions; standard deviations did not exceed <10% of the mean.

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