Optimization of bioconversion process for trehalose production from enzymatic hydrolysis of kudzu root starch using a visualization method

Bioresources and Bioprocessing

Table 1 Uniform design scheme and results of maltose yield rate

Sample	X ₁	X ₂ (U/g starch)	X ₃ (U/g starch)	X ₄ (h)	Factors index YR (%)
1	19	25	0.5	24	76
2	19	30	1	36	92
3	19	35	1.5	48	92
4	20	25	1	48	88
5	20	30	1.5	24	85
6	20	35	0.5	36	86
7	21	25	1.5	36	85
8	21	30	0.5	48	88
9	21	35	1	24	85

X ₁ is the value of DE, X ₂ is the amount of β-amylase, X ₃ is the amount of pullulanase, and X ₄ is saccharification time.
YR maltose yield rate.

Sample	X ₁	X ₂ (U/g starch)	X ₃ (U/g starch)	X ₄ (h)	Factors index YR (%)
1	19	25	0.5	24	76
2	19	30	1	36	92
3	19	35	1.5	48	92
4	20	25	1	48	88
5	20	30	1.5	24	85
6	20	35	0.5	36	86
7	21	25	1.5	36	85
8	21	30	0.5	48	88
9	21	35	1	24	85

Sample	X ₁	X ₂ (U/g starch)	X ₃ (U/g starch)	X ₄ (h)	Factors index YR (%)
1	19	25	0.5	24	76
2	19	30	1	36	92
3	19	35	1.5	48	92
4	20	25	1	48	88
5	20	30	1.5	24	85
6	20	35	0.5	36	86
7	21	25	1.5	36	85
8	21	30	0.5	48	88
9	21	35	1	24	85

Sample	X ₁	X ₂ (U/g starch)	X ₃ (U/g starch)	X ₄ (h)	Factors index YR (%)
1	19	25	0.5	24	76
2	19	30	1	36	92
3	19	35	1.5	48	92
4	20	25	1	48	88
5	20	30	1.5	24	85
6	20	35	0.5	36	86
7	21	25	1.5	36	85
8	21	30	0.5	48	88
9	21	35	1	24	85