Table 1 Construction of synthetic cellulosomes

Chimeric CBM3-CgIT

C. thermocellum scaffoldin

β-glucosidase (CgIT)/(T. brockii)

Fusion of CBM3 from scaffolding protein Cip A into N-terminal region of CgIT

Rice straw

70 %

Waeonukul et al. (2013)

Nanocluster

CdSe–ZnS core–shell quantum dots QDs

Cel A/(C. thermocellum); Cel E/(C. cellulolyticum)

Metal affinity between core–shell QDs and polyhistidine tag

n.d.

n.d.

Tsai et al. (2013)

Mini-cellulosome

Mini-scaffoldin (mini-Cip A)

Endoglucanase/(B. subtilis); processive endoglucanase/(C. thermocellum); cellobiohydrolase/(C. phytofermentans)

High-affinity interaction between three cohesins

Avicel

n.d.

You et al. (2012b)

Nanocluster

Polystyrene nanospheres

Cellulase/(T. viride)

EDC and sulfo-NHS coupling chemistry

n.d.

n.d.

Blanchette et al. (2012)

Hybrid scaffoldin (Scaf 4)

Parental scaffoldin Scaf 4 protein

Cel48F/(–); Cel9G/(–)

Length and composition of inter-module linker connecting C. thermocellum and C. cellulolyticum cohesin modified

Crystalline cellulose

2-fold increase

Molinier et al. (2011)

Functional minicellulosome in Corynebacterium glutamicum

Mini CbpA

Chimeric endoglucanase/(C. cellulovorans)

Fusion of endoglucanase E from C. thermocellum with endoglucanase from C. cellulovorans

CMC

2.8-fold increase

Hyeon et al. (2011)

Scaffoldin-linked exoglucanase

SP1 scaffoldin protein

Cel6B exoglucanase/(T. fusca)

Fusion of chimeric form of enzyme to a cellulosomal dockerin module

n.d.

n.d.

Morais et al. (2010)

Rosettasome

Group II chaperonins from the hyperthermoacidophilic archaeon S. shibatae

Cel9B, Cel9k, Cel9R, Cel48S/(C. thermocellum)

Fusion of cohesin module from C. thermocellum to circular permutant of rosettasome subunit

n.d.

n.d.

Mitsuzawa et al. (2009)

Designer nanosome

Chimeric scaffoldin

Cel A, Cel F/(C. cellulolyticum)

Cohesion–dockerin interaction

n.d.

n.d.

Fierobe et al. (2001)