Fig. 1

Phylogenetic analysis of AbOPH. a Multiple sequence alignment of AbOPH (ZP_09219954) with enzymes in β-lactamase superfamily. Sequences were selected from BLAST in the NCBI (sequence identity >40 %) using input query of OPHC2 from Pseudomonas pseudoalcaligenes (CAE53631), including SmOPH from Stenotrophomonas maltophilia (CCH14104), PoOPH from Pseudomonas oleovorans (4O98_A), PpOPH from Pseudomonas putida (AAR21562), YrOPH from Yersinia rohdei (ZP_04610908), HgOPH from Hylemonella gracilis (ZP_08405683), DpOPH from Desulfobulbus propionicus (YP_004194377), DdOPH from Dickeya dadantii (YP_003884376), PcOPH1 and PcOPH2 from Pectobacterium carotovorum (ZP_03833759; ZP_03833761), MPH from Pseudomonas sp. strain WBC-3 (AAP06948) and VpOPH from Variovorax paradoxus (YP_002945503). Secondary structures are annotated according to the AbOPH structure. Additional residues 206–209 are highlighted by a blue box. Ile211 is indicated with red arrow. Alignment was performed using MUSCLE server and displayed using Esprit (http://espript.ibcp.fr). b Phylogenetic tree analysis of enzymes in β-lactamase superfamily. Phylogenetic tree was built using Discovery Studio 2.5 software. Two major clades are shown in the tree: MPH clade and OPHC2 clade