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Table 1 The comparison of rapamycin production parameters between batches with various inoculation criteria into a 15-L fermenter: liquid seed medium at the ratios of 2, 5 and 10 % (v/v), spore suspension at low and high spore concentrations and mycelium-covered barley inoculated

From: Using the mycelium-covered cereals as an efficient inoculation method for rapamycin fermentation in a 15-L fermenter using Streptomyces hygroscopicus

Inoculation methods

Max. biomass (g/L)

Max. rapamycin (mg/L)

Rapamycin content (mg/g cells)

Rapamycin productivity (mg/L h)

Liquid seed mediuma

 2 %

8.8

229

26 ± 3

1.91

 5 %

9.1

400

41 ± 3

3.04

 10 %

7.2

400

56 ± 5

3.71

Spore suspensionb

 Low SS

8.4

277

33 ± 2

1.92

 High SS

10.4

285

28 ± 3

1.83

Mycelium-barley

14.4

520

36 ± 3

3.61

  1. aInoculation ratio = (seed medium volume/fermentation volume)
  2. bLow SS = 1 × 1010 spore counting per ml of inoculated solution; high SS = 2 × 1010 spore counting per ml of inoculated solution