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Table 1 The comparison of rapamycin production parameters between batches with various inoculation criteria into a 15-L fermenter: liquid seed medium at the ratios of 2, 5 and 10 % (v/v), spore suspension at low and high spore concentrations and mycelium-covered barley inoculated

From: Using the mycelium-covered cereals as an efficient inoculation method for rapamycin fermentation in a 15-L fermenter using Streptomyces hygroscopicus

Inoculation methods Max. biomass (g/L) Max. rapamycin (mg/L) Rapamycin content (mg/g cells) Rapamycin productivity (mg/L h)
Liquid seed mediuma
 2 % 8.8 229 26 ± 3 1.91
 5 % 9.1 400 41 ± 3 3.04
 10 % 7.2 400 56 ± 5 3.71
Spore suspensionb
 Low SS 8.4 277 33 ± 2 1.92
 High SS 10.4 285 28 ± 3 1.83
Mycelium-barley 14.4 520 36 ± 3 3.61
  1. aInoculation ratio = (seed medium volume/fermentation volume)
  2. bLow SS = 1 × 1010 spore counting per ml of inoculated solution; high SS = 2 × 1010 spore counting per ml of inoculated solution