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Table 1 The formula of feed media used in the fed-batch cultures

From: Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

   Asn (mM) Gln (mM) Asn/Gln Asn + Gln (mM)
Total T1 0 0 / 0
T2 30 30 1:1 60
T3 60 60 1:1 120
T4 90 90 1:1 180
T5 120 120 1:1 240
Proportion P1 180 0 / 180
P2 150 30 5:1 180
P3 120 60 2:1 180
P4 90 90 1:1 180
P5 60 120 1:2 180
P6 30 150 1:5 180
P7 0 180 / 180
  FB-L 60 120 1:2 180
FB-H 120 60 2:1 180
FB-N 0 0 / 0