Fig. 3From: Identification of a new thermostable and alkali-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO2 biomineralizationThe effect of temperature on activity of purified LdCA. The assay was carried out at 0 °C with CO2 as a substrate, using 25 mM Tris-HCl (pH 8.3) as buffer. Purified enzyme was preincubated at 20, 30, 40, 50, 60, 70, 80, and 90 °C for 15 min. The enzyme activity measured without any pre-treatment was taken as 100 %Back to article page