Table 2 Conversion and productivity obtained in this study as compared to those reported in literature
From: Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity
Ref. | (Ikeda and Klibanov 1993) | (Yan et al. 1999) | (Ferrer et al. 2000) | (Ferrer et al. 2005) | (Pöhnlein et al. 2014) | This study | |
|---|---|---|---|---|---|---|---|
Substrates | Sugar | Glucose | Glucose | Maltose | Glucose | N-butyryl-glucosamine | Glucose |
Acyl donor | Vinyl acrylate | Palmitic acid methyl ester | Vinyl laurate | Vinyl laurate | Methyl hexanoate | Vinyl laurate | |
Solvent | tert-butanol | Methyl ethyl ketone | 2M2B | 2M2B | 2M2B | 2M2B | |
Enzyme | Pseudomonas sp. lipoprotein lipase | Novozym SP525 | Helvina lanuginosa lipase | Tidestromia. lanuginosus lipase | Novozym 435 | AOL | |
Reaction conditions | Sugar input (M) | 0.1 | 0.01 | 0.12 | 0.3 | 0.04 | 0.3 |
Enzyme amount (mg/ml) | 100 | 1 | 50 | 100 | 5 | 84 | |
Reaction time (h) | 24 | 48 | 24 | 20 | 48 | 5 | |
Reaction temperature (°C) | 45 | 60 | 40 | 40 | 40 | 70 | |
Results | Conversion (%) | 86 | 81 | 65 | 100 | 39 | 64.50 |
Productivity (mmol/L/h) | 3.58 | 0.17 | 3.25 | 15 | 0.325 | 38.7 | |
Specific productivity (μmol/h/g) | 36 | 169 | 65 | 150 | 65 | 461 | |