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Table 1 Experimental design for optimization of CLEA-protease preparation parameters, using faced cantered central composite design FCCCD and response surface methodology RSM, all experiments were done in triplicate

From: Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease

Run Glutaraldehyde (mM) Acetone % (v/v) BSA (mM) Protease specific activity (U/mg protein) CLEA-protease recovered activity (%)a
1 65.00 (0) 60.00 (+1) 0.113 (0) 3.03 22.04
2 80.00 (+1) 60.00 (+1) 0.188 (+1) 1.61 11.71
3 50.00 (−1) 60.00 (+1) 0.188 (+1) 3.01 21.89
4 65.00 (0) 30.00 (−1) 0.113 (0) 2.85 20.73
5 65.00 (0) 45.00 (0) 0.113 (0) 4.57 33.24
6 50.00 (−1) 45.00 (0) 0.113 (0) 3.43 24.95
7 80.00 (+1) 30.00 (−1) 0.188 (+1) 0.96 6.98
8 65.00 (0) 45.00 (0) 0.113 (0) 3.63 26.40
9 80.00 (+1) 30.00 (−1) 0.038 (−1) 1.13 8.22
10 65.00 (0) 45.00 (0) 0.113 (0) 4.14 30.11
11 80.00 (+1) 45.00 (0) 0.113 (0) 2.91 21.16
12 50.00 (−1) 60.00 (+1) 0.038 (−1) 2.16 15.71
13 50.00 (−1) 30.00 (−1) 0.188 (+1) 1.93 14.04
14 50.00 (−1) 30.00 (−1) 0.038 (−1) 1.67 12.15
15 80.00 (+1) 60.00 (+1) 0.038 (−1) 2.73 19.85
16 65.00 (0) 45.00 (0) 0.188 (+1) 2.79 20.29
17 65.00 (0) 45.00 (0) 0.038 (−1) 2.70 19.64
  1. aRecovered activity (%) was calculated according to Eq. 2