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Table 2 Summary of Michaelis–Menten expression for hydrolysis of protease (free and CLEA)

From: Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease

Enzyme Model R 2 K m V max
Free protease Hanes–Woolf 0.997 0.124 0.1173
Lineweaver–Burk 0.966 0.119 0.1164
Eadie–Hofstee 0.937 0.121 0.1169
Hyperbolic regression 0.930 0.127 0.1175
CLEA-protease Hanes–Woolf 0.993 0.258 0.514
Lineweaver–Burk 0.982 0.405 0.577
Eadie–Hofstee 0.871 0.339 0.547
Hyperbolic regression 0.963 0.360 0.550