Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease

Bioresources and Bioprocessing

Table 2 Summary of Michaelis–Menten expression for hydrolysis of protease (free and CLEA)

Enzyme	Model	R ²	K _m	V _max
Free protease	Hanes–Woolf	0.997	0.124	0.1173
	Lineweaver–Burk	0.966	0.119	0.1164
	Eadie–Hofstee	0.937	0.121	0.1169
	Hyperbolic regression	0.930	0.127	0.1175
CLEA-protease	Hanes–Woolf	0.993	0.258	0.514
	Lineweaver–Burk	0.982	0.405	0.577
	Eadie–Hofstee	0.871	0.339	0.547
	Hyperbolic regression	0.963	0.360	0.550