Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

Bioresources and Bioprocessing

Table 4 Experimental planning (2²) and results as function of buffer volume and agitation time

Run	Buffer volume (mL)	Time (min)	Enzyme activity (IU/gds)	Enzyme activity (IU/L)
1	13.79	20	7.50 ± 0.15	1630 ± 5
2	20	10	2.68 ± 0.08	1130 ± 3
3	20	30	4.21 ± 0.15	400 ± 5
4	35	5.86	1.20 ± 0.00	630 ± 2
5^a	35	20	1.72 ± 0.02	110 ± 3
6^a	35	20	1.95 ± 0.18	150 ± 6
7^a	35	20	1.43 ± 0.01	120 ± 2
8	35	34.14	2.34 ± 0.10	200 ± 3
9	50	10	1.25 ± 0.07	80 ± 2
10	50	30	1.63 ± 0.10	100 ± 3
11	56.21	20	3.84 ± 0.00	200 ± 3