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Table 2 Purification of laccase from T. giganteum AGHP

From: Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

Purification step

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Yield (%)

Purification fold

Crude filtrate

8,222,200

254

32,370.86

100

1

Ammonium sulfate precipitation

7,660,050

181.5

42,204.13

93.16

1.30

Dialyzed

2,260,830

45.6

49,579.60

27.49

1.53

Gel permeation chromatography

863,300

8.00

1,07,912.50

10.49

3.33

Ion exchange chromatography

699,225

2.00

3,49,612.50

8.50

10.80