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Table 2 Purification of laccase from T. giganteum AGHP

From: Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

Purification step Total activity (U) Total protein (mg) Specific activity (U/mg) Yield (%) Purification fold
Crude filtrate 8,222,200 254 32,370.86 100 1
Ammonium sulfate precipitation 7,660,050 181.5 42,204.13 93.16 1.30
Dialyzed 2,260,830 45.6 49,579.60 27.49 1.53
Gel permeation chromatography 863,300 8.00 1,07,912.50 10.49 3.33
Ion exchange chromatography 699,225 2.00 3,49,612.50 8.50 10.80