Table 2 Central composite design and response values
Run | Variables | Response | ||
|---|---|---|---|---|
(A) Sucrose (g/L) | (B) Ammonium acetate (g/L) | (C) d-Tryptophan (g/L) | CHA production (mg/L) | |
1 | 100 | 3.5 | 4.283 | 53.29 ± 1.13 |
2 | 100 | 3.5 | 4.283 | 53.92 ± 0.73 |
3 | 100 | 2.66 | 4.283 | 46.23 ± 2.51 |
4 | 100 | 3.5 | 2.603 | 43.21 ± 0.77 |
5 | 66.363 | 3.5 | 4.283 | 44.92 ± 1.16 |
6 | 100 | 3.5 | 4.283 | 51.79 ± 2.05 |
7 | 100 | 3.5 | 4.283 | 52.82 ± 0.12 |
8 | 100 | 3.5 | 4.283 | 53.62 ± 1.71 |
9 | 80 | 3 | 3.284 | 43.74 ± 0.95 |
10 | 120 | 4 | 3.284 | 57.82 ± 0.21 |
11 | 80 | 4 | 3.284 | 43.23 ± 2.55 |
12 | 133.634 | 3.5 | 4.283 | 45.38 ± 1.42 |
13 | 120 | 4 | 5.282 | 47.83 ± 0.73 |
14 | 80 | 4 | 5.282 | 44.93 ± 1.34 |
15 | 120 | 3 | 5.282 | 44.94 ± 0.95 |
16 | 100 | 3.5 | 5.963 | 45.78 ± 2.11 |
17 | 120 | 3 | 3.284 | 39.78 ± 2.76 |
18 | 100 | 4.341 | 4.283 | 59.42 ± 0.22 |
19 | 80 | 3 | 5.282 | 47.94 ± 0.94 |
20 | 100 | 3.5 | 4.283 | 52.86 ± 1.55 |