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Table 4 Physicochemical characterization of the biosurfactant produced by K. pneumoniae IVN51

From: Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Material Surface tension (mN/m) Emulsification assay (@400 nm) Emulsification index (E 24)  % Tilting glass slide test Oil-spreading test (mm2)a
DH2O 71 ± 0.9
Partially purified biosurfactant 31 ± 0.4 0.4363 ± 0.001 60 ± 2.0 + 254.3 ± 4.2
SDS 35 ± 0.8 0.6113 ± 0.004 70.0 ± 1.6 + 283.4 ± 3.14
  1. All values represent mean ± SD for triplicate cultures
  2. SDS Sodium dodecyl sulphate (positive control); + positive; − negative; DH 2 O distilled water (negative control)
  3. a Surface area