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Table 4 Physicochemical characterization of the biosurfactant produced by K. pneumoniae IVN51

From: Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Material

Surface tension (mN/m)

Emulsification assay (@400 nm)

Emulsification index (E 24)  %

Tilting glass slide test

Oil-spreading test (mm2)a

DH2O

71 ± 0.9

–

–

–

–

Partially purified biosurfactant

31 ± 0.4

0.4363 ± 0.001

60 ± 2.0

+

254.3 ± 4.2

SDS

35 ± 0.8

0.6113 ± 0.004

70.0 ± 1.6

+

283.4 ± 3.14

  1. All values represent mean ± SD for triplicate cultures
  2. SDS Sodium dodecyl sulphate (positive control); + positive; − negative; DH 2 O distilled water (negative control)
  3. a Surface area