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Table 5 Preliminary result showing the class of the biosurfactant produced

From: Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Biosurfactant test Biosurfactant aimed at detecting Result
Biuret test Lipopeptide biosurfactant
CTAB/methylene-blue agar test Rhamnolipid
Phosphate test Phospholipids +a
  1. + positive; − negative
  2. a Formation of yellow color, which was followed by the slow formation of a fine yellow precipitate, indicated the presence of phospholipid biosurfactant