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Table 1 List of degeneration codon used in each position of different libraries

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library Degenerate codon Alphabets Library Degenerate codon Alphabets
Library A
(Site 1)
I80: VDT
V83: SDT
L114: SDT
I116: VDT
VDT: Leu, Ile, Val, His, Asn, Asp, Arg, Ser, Gly;
SDT: Leu, Val, His, Asp, Arg, Gly
BWT: Phe, Tyr, Leu, Val, His, Asp
Library C
(site 1)
I80: GBT/HTT
V83: GBT/HTT
L114: GBT/HTT
I116: GBT/HTT
GBT: Val, Ala, Gly
HTT: Phe, Leu, Ile
Library B
(Site 2)
L35: SDT
L74: SDT
L103: SDT
F134: BWT
Library D
(Site 2)
L35: GBT/HTT
L74: GBT/HTT
L103: GBT/HTT
F134: GBT/HTT