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Table 1 List of degeneration codon used in each position of different libraries

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library

Degenerate codon

Alphabets

Library

Degenerate codon

Alphabets

Library A

(Site 1)

I80: VDT

V83: SDT

L114: SDT

I116: VDT

VDT: Leu, Ile, Val, His, Asn, Asp, Arg, Ser, Gly;

SDT: Leu, Val, His, Asp, Arg, Gly

BWT: Phe, Tyr, Leu, Val, His, Asp

Library C

(site 1)

I80: GBT/HTT

V83: GBT/HTT

L114: GBT/HTT

I116: GBT/HTT

GBT: Val, Ala, Gly

HTT: Phe, Leu, Ile

Library B

(Site 2)

L35: SDT

L74: SDT

L103: SDT

F134: BWT

Library D

(Site 2)

L35: GBT/HTT

L74: GBT/HTT

L103: GBT/HTT

F134: GBT/HTT