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Table 3 Best LEH variants of libraries C and D as catalysts in the hydrolytic desymmetrization of epoxide 1 with formation of (R,R)- or (S,S)-2

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library Code Mutations Favored enantiomer ee % c %
C WT   (S,S) 2 99
SZ57 I80 V/L114 V (R,R) 75 99
SZ197 I80 V (R,R) 72 93
SZ503 I80 V/V83I/L114 V (R,R) 80 97
SIF01 I80F/V83I/L114 V (R,R) 72 94
SZ501 I80F/V83I/L114 V/I116G (S,S) 97 98
SZ502 I80F/V83I/L114 V/I116 V (S,S) 98 95
SZ504 I80F/V83I/L114I/I116 V (S,S) 95 98
SZ546 L114 V/I116 V (S,S) 68 95
D TOQ-PA-197-04 L103I/F134L (R,R) 40 51
TOQ-PA-197-06 L74F/L103I/F134L (R,R) 55 35
TOQ-PA-197-01 L35 V/L74F/L103 V (S,S) 36 92
TOQ-PA-197-05 L35 V/L74F (S,S) 38 67
TOQ-PA-197-07 L35A/L74F/L103I (S,S) 38 62