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Table 4 Best LEH variants of library CD and DC for catalytic desymmetrization of epoxide 1

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library Code Mutations Favored enantiomer ee % c %
CD SZ503 I80 V/V83I/L114 V (template) (R,R) 80 97
TOQ-PA-218-08 L35G/I80 V/V83I/L103I/L114 V (R,R) 80 90
TOQ-PA-218-24 L74 V/I80 V/V83I/L114 V (R,R) 67 91
TOQ-PA-218-32 L74I/I80 V/V83I/L114 V (R,R) 79 97
DC1 TOQ-PA-197-06 L74F/L103I/F134L (template) (R,R) 55 35
TOQ-PB-280-07 L74F/V83I/L103I/L114A/I116 V/F134L (R,R) 40 55
TOQ-PB-280-09 L74F/I80 V/L103I/F134L (R,R) 61 41
DC2 TOQ-PA-197-05 L35 V/L74F (template) (R,R) 38 67
SZ274 L35 V/L74F/I80 V/V83I/L114 V (R,R) 38 63
SZ277 L35 V/L74F/I80G/V83I/L114 V (R,R) 35 46
SZ278 L35 V/L74F/I80F/V83I/I116 V (S,S) 83 52
SZ280 L35 V/L74F/I80F/I116 V (S,S) 80 70
SZ283 L35 V/L74F/L114 V/I116 V (S,S) 87 80
SZ284 L35 V/L74F/I80F/I116 V (S,S) 81 65
SZ285 L35 V/L74F/L114 V/I116 V (S,S) 86 83
SZ288a L35 V/L74F/V83T/L114 V/I116 V (S,S) 90 66
SZ290 L35 V/L74F/V83I/L114 V/I116 V (S,S) 84 67
  1. aThe mutation of V83T of SZ288 was introduced randomly in the PCR procedure, which was not designed in library DC2