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Bioresources and Bioprocessing

Table 4 Best LEH variants of library CD and DC for catalytic desymmetrization of epoxide 1

From: Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Library

Code

Mutations

Favored enantiomer

ee %

c %

CD

SZ503

I80 V/V83I/L114 V (template)

(R,R)

80

97

TOQ-PA-218-08

L35G/I80 V/V83I/L103I/L114 V

(R,R)

80

90

TOQ-PA-218-24

L74 V/I80 V/V83I/L114 V

(R,R)

67

91

TOQ-PA-218-32

L74I/I80 V/V83I/L114 V

(R,R)

79

97

DC1

TOQ-PA-197-06

L74F/L103I/F134L (template)

(R,R)

55

35

TOQ-PB-280-07

L74F/V83I/L103I/L114A/I116 V/F134L

(R,R)

40

55

TOQ-PB-280-09

L74F/I80 V/L103I/F134L

(R,R)

61

41

DC2

TOQ-PA-197-05

L35 V/L74F (template)

(R,R)

38

67

SZ274

L35 V/L74F/I80 V/V83I/L114 V

(R,R)

38

63

SZ277

L35 V/L74F/I80G/V83I/L114 V

(R,R)

35

46

SZ278

L35 V/L74F/I80F/V83I/I116 V

(S,S)

83

52

SZ280

L35 V/L74F/I80F/I116 V

(S,S)

80

70

SZ283

L35 V/L74F/L114 V/I116 V

(S,S)

87

80

SZ284

L35 V/L74F/I80F/I116 V

(S,S)

81

65

SZ285

L35 V/L74F/L114 V/I116 V

(S,S)

86

83

SZ288a

L35 V/L74F/V83T/L114 V/I116 V

(S,S)

90

66

SZ290

L35 V/L74F/V83I/L114 V/I116 V

(S,S)

84

67

  1. aThe mutation of V83T of SZ288 was introduced randomly in the PCR procedure, which was not designed in library DC2
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