Table 1 Strains and plasmids used in this study
Strains or plasmids | Genotype and/or relevant characteristics | Source or reference |
|---|---|---|
Strains | ||
Escherichia coli | ||
DH5α | Host for general | |
ET12567/pUZ8002 | Donor strain for conjugation between E. coli and Streptomyces | Invitrogen Lab. collection |
S. aureofaciens | ||
DT1 | producer of S. aureofaciens Industrial 6-DCT-producing strain | Lab. collection |
A6-9 | 6-DCT high-yield strains mutated from DT1 | Lab. collection |
ΔctcH | The deletion mutant of ctcH derived from DT1 | This study |
ΔctcJ | The deletion mutant of ctcJ derived from DT1 | This study |
ctcH+ | ctcH gene-duplicated derivative of DT1, with genotype PermE*-ctcH gene | This study |
ctcJ+ | ctcJ gene-duplicated derivative of DT1, with genotype PermE*-ctcJ gene | This study |
ctcH-ctcJ+ | ctcH and ctcJ gene-duplicated derivative of DT1, with genotype PermE*-ctcH-ctcJ gene | This study |
Plasmids | ||
pOJ260 | Non-replicating vector, Apms | Lab. collection |
pIB139 | attφC31, oriT, Apms, PermE* | Lab. collection |
pOJ260-neo | pOJ260 derivative carrying a 1145 bp DNA fragment containing the neo gene, Kans, Apms | This study |
pOJ260-neo-H | pOJ260-neo containing a 959 bp upstream fragment and a 889 bp downstream fragment of ctcH, Kans, Apms | This study |
pOJ260-neo-J | pOJ260-neo containing a 911 bp upstream fragment and a 1021 bp downstream fragment of ctcJ, Kans, Apms | This study |
pIB139-H | pIB139 derivative carrying a 719 bp DNA fragment containing the ctcH gene | This study |
pIB139-J | pIB139 derivative carrying a 763 bp DNA fragment containing the ctcJ gene | This study |
pIB139-H-J | pIB139 derivative carrying a 763 bp and a 719 bp DNA fragments containing the ctcJ and ctcH gene | This study |