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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Penicillin acylase-catalyzed synthesis of N-bromoacetyl-7-aminocephalosporanic acid, the key intermediate for the production of cefathiamidine

Fig. 4

Effect of substrate molar ratio (a), pH (b), and temperature (c) on the synthesis of N-bromoacetyl-7-ACA. Reaction conditions for part a: 40 mmol/L 7-ACA, methyl bromoacetate of the designated concentration, 3 U/mL PGA-750, 10 mL phosphate buffer (100 mmol/L, pH 7.5), 200 r/min, 20 °C; reaction conditions for part b: 40 mmol/L 7-ACA, 120 mmol/L methyl bromoacetate, 3 U/mL PGA-750, 10 mL buffer (100 mmol/L, pH 7.0–8.0, phosphate buffer; pH 8.5–9.0, Tris–HCl buffer), 200 r/min, 20 °C; reaction conditions for part c: 40 mmol/L 7-ACA, 120 mmol/L methyl bromoacetate, 3 U/mL PGA-750, 10 mL phosphate buffer (100 mmol/L, pH 7.5), 200 r/min, the designated temperature

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