Fig. 7From: Penicillin acylase-catalyzed synthesis of N-bromoacetyl-7-aminocephalosporanic acid, the key intermediate for the production of cefathiamidineOperational stability of the immobilized enzyme PGA-750. Reaction conditions: 50 mmol/L 7-ACA, 150 mmol/L methyl bromoacetate, 4 U/mL PGA-750, 10 mL phosphate buffer (100 mmol/L, pH 7.5), 200 r/min, 20 °C. The reaction time of each batch was 2 h. The relative yield of the first batch was defined as 100%Back to article page