Table 1 Strains, plasmids, and primers used in this study
From: Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin
Strains, plasmids and primers | Description | Source |
|---|---|---|
E.coli S17-1(λ pir) | recA thi pro hsdR − M + RP4::2-Tc::Mu::Km Tn7 lysogenized with λ pir phage | Laboratory stock |
S. marcescens MG1 | Wild type, TcrApr | Laboratory stock |
MG12 | S. marcescens MG1 ΔslaC | This study |
MG13 | S. marcescens MG1 ΔgldA | This study |
MG14 | S. marcescens MG1 ΔslaCΔgldA | This study |
MG15 | S. marcescens MG1 ΔslaCΔgldA/pACPC-slaR | This study |
MG16 | S. marcescens MG1 ΔslaCΔgldA/pACPAB-slaR | This study |
MG17 | S. marcescens MG1 ΔslaCΔgldA/pACPR-slaR | This study |
pUTKm1 | Apr Kmr oriR6 K oriTRP4 | (Lorenzo et al. 1990) |
pUT-slaC | pUTKm1 containing a 1798 bp deletion of slaC | This study |
pUT-gldA | pUTKm1 containing a 1804 bp deletion of gldA | This study |
pACYC184 | CmR | (Sun et al. 2015) |
pACPC-slaR | pACYC184 containing slaR under the promotor of slaC | This study |
pACPA-slaR | pACYC184 containing slaR under the promotor of slaA | This study |
pACPR-slaR | pACYC184 containing slaR under the promotor of slaR | This study |
slaC-F1 | GTggtaccCATGCGGCAAGGAGCGCCATC | This study |
slaC-F2 | GGCCTGTGCGTTAACGCGAGACCTCCTCCATGTGAAC | This study |
slaC-R1 | GTTCACATGGAGGAGGTCTCGCGTTAACGCACAGGCC | This study |
slaC-R2 | GTgagtactCAGCCGCATCAGCCGCTAC | This study |
gldA-F1 | TTCggtaccGGTTGCGTTCAATGATGATG | This study |
gldA-F2 | CTCCCTACAAGGATCCGGTTTACCCTTGGGGCGCGGTGTGC | This study |
gldA-R1 | GCACACCGCGCCCCAAGGGTAAACCGGATCCTTGTAGGGAG | This study |
gldA-R2 | GCTagatctCTGCATGCTGGTCTGCTTGG | This study |
P C-1 | GTtctagaTCGCGGCCGCCTGCGGGC | This study |
P C-2 | GTaagcttGAGACCTCCTCCATGTG | This study |
P A-1 | GCtctagaAAAACGTAATATACGTTT | This study |
P A-1 | GCaagcttCTGACTGAGGAGGTGGTC | This study |
P R-1 | GCtctagaCTGACTGAGGAGGTGGTCGC | This study |
P R-2 | GCaagcttTTTTGCATTATATGCAAA | This study |
slaR-1 | GCaagcttATGAATGACGCACGCTATG | This study |
slaR-2 | GCggatccAATAGGGGTCGACCCGCCAA | This study |