Table 2 The Plackett–Burman design for screening variables in (3R)-AC production
From: Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin
Factors (g/l) | Variables | Low level (−1) | High level (+1) | Effect | Coef. | t value | p value |
|---|---|---|---|---|---|---|---|
Sucrose | X 1 | 60 | 90 | 3.310 | 0.322 | 10.29 | 0.001 |
Yeast extract | X 2 | 15 | 25 | 0.287 | 0.322 | 0.89 | 0.423 |
Sodium citrate | X 3 | 5 | 15 | 0.602 | 0.322 | 1.87 | 0.135 |
Sodium acetate | X 4 | 1 | 3 | −1.012 | 0.322 | −3.14 | 0.035 |
NH4H2PO4 | X 5 | 0.2 | 0.5 | 0.628 | 0.322 | 1.95 | 0.123 |
MgSO4 | X 6 | 0.2 | 0.5 | −0.057 | 0.322 | −0.18 | 0.869 |
MnSO4 | X 7 | 0.02 | 0.05 | −0.842 | 0.322 | −2.62 | 0.059 |