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Bioresources and Bioprocessing

Table 2 Treatments of the fractionated design with a central point

From: Hydrolysis of carotenoid esters from Tagetes erecta by the action of lipases from Yarrowia lipolytica

Factor

A

B

C

D

E

F

G

No.

Design coordinate

G/L

ml/L

1

−−++−−+

10

3

2

2

1

0.8

4

2

+++++++

20

5

2

2

2

2

4

3

−++−+−−

10

5

2

1.4

2

0.8

2

4

−+−+−+−

10

5

1

2

1

2

2

5

−−−−+++

10

3

1

1.4

2

2

4

6

+−−++−−

20

3

1

2

2

0.8

2

7

++−−−−+

20

5

1

1.4

1

0.8

4

8

0000000

15

4

1.5

1.7

1.5

1.4

3

9

+−+−−+−

20

3

2

1.4

1

2

2

  1. + and − represent high and low level, respectively, for factors ABCDEFG. 0 stands for a medium level which results from the average of the maximum and minimum value
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