Fig. 5From: Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775Monitoring culture parameter effect on lipid accumulation in Y. lipolytica JMY775 reviled by online flow cytometry and fluorescent microscopy analyses. a Results corresponding to the samples displayed in panel b (FL3-A: red fluorescence intensity proportional to Nile-red staining; SSC-A: side scatter intensity). b Microscopy images (40×) showing the lipid accumulation in the yeast for different bioreactor operating conditions (control bioreactor: culture operated with dissolved oxygen and pH controls; dissolved oxygen bioreactor: culture operated with dissolved oxygen limitation; and pH bioreactor: bioreactor operated without pH control). For each condition, cells have been sampled at different time intervals and stained with Nile-red Back to article page