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Table 1 Differences of the bi-enzymatic system with immobilized enzymes and free enzymes

From: Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase

The bi-enzymatic system Total activity of SMDH (U) Total activity of laccase (U) Substrate concentration (mM) Reaction time (h) Reaction velocity (μmol/h∙U)
The free bi-enzymatic system 49.0 25.0 10.0 12.0 0.85
The immobilized bi-enzymatic system 12.9 6.50 30.0 10.0 11.6