Table 1 Differences of the bi-enzymatic system with immobilized enzymes and free enzymes
The bi-enzymatic system | Total activity of SMDH (U) | Total activity of laccase (U) | Substrate concentration (mM) | Reaction time (h) | Reaction velocity (μmol/h∙U) |
|---|---|---|---|---|---|
The free bi-enzymatic system | 49.0 | 25.0 | 10.0 | 12.0 | 0.85 |
The immobilized bi-enzymatic system | 12.9 | 6.50 | 30.0 | 10.0 | 11.6 |