An efficient multi-stage fermentation strategy for the production of microbial oil rich in arachidonic acid in Mortierella alpina

Bioresources and Bioprocessing

Table 2 Comparison of parameters from different ARA fermentation strategies

	Fermentation strategy			Rate of increase^a
	Batch	Fed-batch	Multi-stage	Rate of increase^a
DCW (g/L)	31.06	36.08	41.41	14.77%
Mycelial specific growth rate (g/L d⁻¹)	4.78	6.01	5.92	−1.50%
Lipids (g/L)	17.63	17.09	22.17	25.75%
Fermentation duration (d)	6.5	6	7.5	7.69%
Total glucose (g/L)	80	80	100	25%
Glucose consumption rate (g/L h⁻¹)	0.51	0.56	0.76	35.71%
ARA contents (%)	36.30	42.81	61.05	42.61%
ARA yield (g/L)	6.40	10.01	13.53	35.16%
ARA productivity (g/L d⁻¹)	0.98	1.67	1.81	7.78

^aThis value represents the corresponding data from the multi-stage strategy divided by the highest respective value from either the batch or fed-batch fermentation