Table 1 The Plackett–Burman design and effects of nine factors for SrtA production
Factors | Code | High level (+1) | Low level (−1) | Coefficient | F value | P value |
|---|---|---|---|---|---|---|
Glycerol (g/L) | A | 1.0 | 0.1 | −162.08 | 27.97 | 0.03 |
Tryptone (g/L) | B | 4.0 | 1.0 | −243.91 | 63.35 | 0.02 |
Yeast extract (g/L) | C | 4.0 | 1.0 | −201.77 | 43.35 | 0.02 |
PBS (%) | D | 20.0 | 5.0 | −17.02 | 0.31 | 0.63 |
MOPS (g/L) | E | 1.5 | 0.5 | −85.82 | 7.84 | 0.11 |
Induction occasion (OD600) | F | 1.0 | 0.4 | 113.59 | 13.74 | 0.07 |
IPTG (mM) | G | 1.5 | 0.2 | 33.43 | 1.19 | 0.39 |
Inoculation (%) | H | 5.0 | 1.0 | −4.80 | 12.83 | 0.89 |
Loading volume (%) | I | 20.0 | 6.0 | −109.76 | 0.03 | 0.07 |