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Table 2 The Box–Behnken design matrix and experimental results

From: Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations

Runs

A

B

C

SrtA production (U/mg DCW)

1

0

0

0

1491.1

2

0

−1

+1

1394.1

3

+1

−1

0

971.6

4

+1

0

+1

912.2

5

−1

−1

0

1418.7

6

0

0

0

1468.3

7

0

+1

+1

1505.3

8

−1

0

−1

1666.6

9

0

+1

−1

1588.2

10

+1

0

−1

988.1

11

−1

+1

0

1427.9

12

+1

+1

0

974.5

13

0

0

0

1464.8

14

0

−1

−1

1566.6

15

−1

0

+1

1457.3

16

0

0

0

1492.4

17

0

0

0

1482.4