Table 3 The enzyme activity and yield of SrtA performed in the optimized conditions (optimized medium, 30 °C for 8 h) and initial conditions (LB medium, 37 °C for 4 h) with shaking-flask fermentation and at 7-L fermentor level
Samples | Shaking-flask level | 7-L fermentor level | ||
|---|---|---|---|---|
Activity (U/mg DCW) | Yield (mg/L) | Activity (U/mg DCW) | Yield (mg/L) | |
Initial conditions | 855.2 ± 95.1 | 44.2 ± 2.1 | 423.9 ± 8.8 | 52.1 ± 3.9 |
Predicted values | 1674 | 155 | – | – |
Optimized conditions | 1732.3 ± 122.4 | 188.1 ± 18.3 | 2458.4 ± 115.9 | 232.4 ± 21.1 |