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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

Fig. 3

a SDS-PAGE analysis of recombinant MCO generated from (left) pET28a-lacB at 24 h, (middle) pET32a-lacB at 24 h, and (right) pET22b-lacB at 14 h transformed in E. coli BL21(DE3) cells. Samples used are W, whole cell; S, supernatant; P, pellet; and M, periplasm; where subscript of a, b and instead of induction by IPTG with Cu, IPTG and none of them. Mw means molecular weight. The arrows indicated the overexpression of MCO. b Crude MCO activity in whole cell lysates, soluble and cell pellet from recombinant (left) pET28a, (middle) pET32a and (right) pET22b at different pHs

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