Strains, plasmid, or primer | Description |
---|---|
E. coli strains | |
DH5α | F− recA1 endA1 hsdR17(r − k m + k ) supE44 thi-1 gyrA relA1 |
BL21(DE3) | F− ompT hsdS B (r − B m − B ) gal dcm (DE3) |
Plasmids | |
pET-32a | Ampr T7 promoter trxA-tag His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector |
pET-28a | Kanr T7 promoter His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector |
pET-22b | Ampr T7 promoter pelB His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector |
Primersa | |
ZMd-lacB-XhoI-r | 5′-atCTCGAGTTTACTCACAGTAAAACCCG |
ZMd-lacB-NcoI-f | 5′-taCCATGGATCAAAGTAACACGCCTTC |