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Table 1 List of strains, plasmids, and oligonucleotide primers in this study

From: Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

Strains, plasmid, or primer Description
E. coli strains
 DH5α F recA1 endA1 hsdR17(r k m k + ) supE44 thi-1 gyrA relA1
 BL21(DE3) F ompT hsdS B (r B m B ) gal dcm (DE3)
Plasmids
 pET-32a Ampr T7 promoter trxA-tag His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector
 pET-28a Kanr T7 promoter His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector
 pET-22b Ampr T7 promoter pelB His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector
Primersa
 ZMd-lacB-XhoI-r 5′-atCTCGAGTTTACTCACAGTAAAACCCG
 ZMd-lacB-NcoI-f 5′-taCCATGGATCAAAGTAACACGCCTTC
  1. aNucleotides shown in underline represented the restriction sites