Skip to main content

Table 1 List of strains, plasmids, and oligonucleotide primers in this study

From: Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

Strains, plasmid, or primer

Description

E. coli strains

 DH5α

F recA1 endA1 hsdR17(r k m + k ) supE44 thi-1 gyrA relA1

 BL21(DE3)

F ompT hsdS B (r B m B ) gal dcm (DE3)

Plasmids

 pET-32a

Ampr T7 promoter trxA-tag His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector

 pET-28a

Kanr T7 promoter His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector

 pET-22b

Ampr T7 promoter pelB His-tag T7 terminator lacI f1 pBR322 origin E. coli expression vector

Primersa

 ZMd-lacB-XhoI-r

5′-atCTCGAGTTTACTCACAGTAAAACCCG

 ZMd-lacB-NcoI-f

5′-taCCATGGATCAAAGTAACACGCCTTC

  1. aNucleotides shown in underline represented the restriction sites