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Table 1 Selection of the recombinant enzymes for catalyzing DKTP reduction to (S)-DHTP

From: Enhancement of asymmetric bioreduction of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine to corresponding (S)-enantiomer by fusion of carbonyl reductase and glucose dehydrogenase

Enzyme

GenBank ID

Source

Product configuration/yield (%)

References

S1

AB036927

Candida magnoliae

–

Kizaki et al. (2001)

C1

AB084515

C. parapsilosis

–

Kataoka et al. (2004)

C2

AB084516

C. parapsilosis

–

Kataoka et al. (2004)

ADHR

AY267012

Lactobacillus kefiri

–

Weckbecker and Hummel (2006)

CR2

AB183149

Kluyveromyces marxianus

S/91.31 ± 0.45

Kataoka et al. (2006)

CR4

E59061

K. aestuarii

–

Yamamoto et al. (2004)

KRD

AF178079

Zygosaccharomyces rouxii

S/55.18 ± 0.25

Costello et al. (2000)

OYE

AB126227

K. marxianus

–

Kataoka et al. (2002)

RCR

DQ295067

C. parapsilosis

–

Nie et al. (2008)

SCR

DQ675534

C. parapsilosis

–

Nie et al. (2011)

SCR1

FJ939565

C. parapsilosis

–

Nie et al. (2011)

SCR3

FJ939564

C. parapsilosis

–

Nie et al. (2011)

CPAR1

JX512911

C. parapsilosis

–

Guo et al. (2014)

CPAR2

JX512912

C. parapsilosis

–

Guo et al. (2014)

CPAR3

JX512913

C. parapsilosis

–

Guo et al. (2014)

CPAR4

JX512915

C. parapsilosis

–

Guo et al. (2014)

CPAR5

JX512916

C. parapsilosis

–

Guo et al. (2014)

CPAR6

JX512917

C. parapsilosis

–

Guo et al. (2014)

CPAR7

JX512918

C. parapsilosis

–

Guo et al. (2014)

CPAR8

JX512919

C. parapsilosis

–

Guo et al. (2014)

  1. All reactions were carried out in 2 mL Tris–HCl buffer (0.05 mol L−1, pH 8.0) comprising 1 g L−1 DKTP with addition of 10 g L−1 glucose and 0.02 mmol L−1 NADP+ at 30 °C and 200 rpm for 12 h. All the results were the average of three parallel replicates