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Bioresources and Bioprocessing

Table 2 Kinetic parameters of the fusion enzyme CR2-L-GDH towards DKTP and glucose, respectively

From: Enhancement of asymmetric bioreduction of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine to corresponding (S)-enantiomer by fusion of carbonyl reductase and glucose dehydrogenase

Substrate

V max (μmol min−1 mg−1)

K m (mmol L−1)

k cat (s−1)

k cat/K m (L mol−1 s−1)

DKTP

6.75 ± 0.15

0.32 ± 0.05

4.35 ± 0.07

1.37 × 104

Glucose

2.58 ± 0.12

0.47 ± 0.04

1.66 ± 0.12

3.54 × 103

  1. All reactions for the calculation of kinetic parameters were carried out in 0.1 mol L−1 TEA buffer (pH 8.0) at 30 °C. For activity assay to calculate kinetic parameters, the cofactor of NADPH or NADP+ was added in the reaction mixture at saturated concentration towards the enzyme. All the results were the average of three parallel replicates
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