Table 3 Effect of NADP⁺ concentration on CR2-L-GDH catalyzing asymmetric synthesis of (S)-DHTP

From: Enhancement of asymmetric bioreduction of N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine to corresponding (S)-enantiomer by fusion of carbonyl reductase and glucose dehydrogenase

NADP⁺ (mmol L⁻¹)	Optical purity (% e.e.)	Yield (%)	TTN
0.2	>99.9	92.75 ± 0.21	501
0.1	>99.9	94.87 ± 0.15	1024
0.05	>99.9	83.35 ± 0.23	1799
0.02	>99.9	72.46 ± 0.18	3911
0.01	>99.9	64.64 ± 0.12	6977
0.005	>99.9	50.45 ± 0.28	10,891

All reactions were carried out in 10 mL TEA buffer (0.1 mol L⁻¹, pH 8.4) comprising 20 g L⁻¹ DKTP with addition of 100 g L⁻¹ glucose at 40 °C and 200 rpm for 10 h. All the results were the average of three parallel replicates

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