Table 4 Comparison of (S)-DHTP production efficiency between free CR2 and GDH and fusion enzyme CR2-L-GDH
Enzyme | Substrate concentration (g L−1) | Yield (%) | Optical purity (% e.e.) |
|---|---|---|---|
CR2 and GDH | 10 | 90.43 ± 0.37 | >99.9 |
CR2 and GDH | 20 | 47.25 ± 0.26 | >99.9 |
CR2-L-GDH | 20 | 97.66 ± 0.16 | >99.9 |