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Table 2 Effect of different metallic ions, surfactants, chelating agents, and inhibitors on xylanase activity

From: Biochemical properties of a new thermo- and solvent-stable xylanase recovered using three phase partitioning from the extract of Bacillus oceanisediminis strain SJ3

Chemical additives

Concentration

Relative enzyme activity (%)a

Control

–

100 ± 2.5

Mg2+ (MgCl2)

5 mM

106 ± 2.6

Ca2+ (CaCl2)

5 mM

138 ± 4.1

Fe2+ (FeSO4)

5 mM

88 ± 2.2

K+ (KCl)

5 mM

98 ± 2.4

Cu2+ (CuCl2)

5 mM

92 ± 2.3

Na+ (NaCl)

5 mM

94 ± 2.3

Mn2+ (MnCl2)

5 mM

99 ± 2.5

Cd2+ (CdCl2)

5 mM

83 ± 2.0

Zn2+ (ZnCl2)

5 mM

95 ± 2.3

Hg2+ (HgCl2)

5 mM

20 ± 0.6

Triton X-100

1%

93 ± 2.3

SDS

1%

87 ± 2.2

EDTA

5 mM

88 ± 2.2

β-Mercaptoethanol

20 mM

86 ± 2.2

  1. Xylanase activity measured in the absence of any chemical additives was taken as control (100%). The non-treated and dialyzed enzyme was considered as 100% for metallic ion assay. Residual activity was measured at pH 7.0 and 55 °C
  2. aValues represent means of three independent replicates, and ±standard errors are reported