From: Recent progress on deep eutectic solvents in biocatalysis
Catalyst | DES | Substrate | Comments | References |
---|---|---|---|---|
Haloalkane dehalogenases | ChCl/EG (1:2) | 1-Iodohexane | Improved enzyme thermostability and substrate solubility | Stepankova et al. (2014) |
Benzaldehyde lyase | ChCl/Gly (1:2) | Butyraldehyde; valeraldehyde; benzaldehyde; 2-furaldehyde | Improved e.e. | Maugeri and DomÃnguez de MarÃa (2014) |
Phospholipase D | ChCl/EG (1:2) | Phosphatidylcholine with l-serine | >90% yield of phosphatidylserine | Yang and Duan (2016) |
Diglycosidase | ChCl/Gly (1:2); ChCl/EG (1:2) | Hesperidin | Enhanced substrate solubility | Weiz et al. (2016) |
Horseradish peroxidase; cytochrome c | ChCl or EAC with U, Gly and EG (1:1.5,1:2) | Guaiacol | Enhancing the functional stability of protein | Papadopoulou et al. (2016) |
Chondroitinases ABCI | ChCl/Gly (1:2) | Chondroitin | Improving thermal stability remarkably | Daneshjou et al. (2017) |
β-d-glucosidase | ChCl/EG (2:1) | Daidzin | Application of DES in the synthesis of bioactive compound | Cheng and Zhang (2017) |
Bovine liver catalase | ChCl/EG (1:2) | Hydrogen peroxide | DES could change the Km and Kcat of enzyme in DES-containing solution. Structure test found that the 3D structure was influenced by the addition of DES | Harifi-Mood et al. (2017) |