Fig. 2
From: Photocontrolled reversible self-assembly of dodecamer nitrilase
Expressions of BNAS and SspB proteins in E. coli BL21 (DE3) cells. a SDS-PAGE image of the cells expressing BNAS and SspB at 20 h after IPTG addition. Lanes 1 marker; lane 2 BNAS; lane 3 SspB; lane 4 control. The arrow shows the destination band. b SDS-PAGE analyses of purified BNAS and SspB in vitro. Lanes 1 and 4 marker; lane 2 purified BNAS; lane 3 purified SspB. c Comparison of the enzyme activities of BCNIT and BNAS in vitro. The determination of nitrilase activity was performed at 30 °C for 30 min with 10 µM FMN under 470-nm blue light illumination. Under the experimental conditions, the relative activity was expressed as a percentage of the maximum activity. Error bars represent the standard error of three replicates