Table 1 Primers used for cloning and transcript amplification in this study
From: Photocontrolled reversible self-assembly of dodecamer nitrilase
Primer | Sequence (5′–3′) |
|---|---|
P1 | GGAATTCCATATGGAATACAAATCCTC |
R1 | CCGCTCGAGTTATTCATCGTAGATTTCTTCAG |
P2 | GGAATTCCATATGACCATCAATCACCCG |
R2 | GACTAGTCGAGCCACCGCCACCAGCGGGTGTGACGCGC |
P3 | GGACTAGTGGCGGTGGCGGATCTTTAGCCACTACTTTAGAAAGG |
R3 | ATAAGAATGCGGCCGCAAAATAATTTTCATCATTAG |
P4 | AGGTGGCTCTACTAGTGGCGGAGGTGGCTCTGTG |
R4 | CACCTCCGCCACTAGTCGATCCGCCACCGCCGTC |
P5 | AGGTGGCTCTACTAGTGGCGGTGGCGGATCTGGC |
R5 | CACCTCCGCCACTAGTAGAGCCACCTCCGCCGCT |