Table 2 Mutation and screening results for each round of ARTP mutagenesis
Iterative round | R M (%) | R P (%) | Average \(\Delta A_{525}\) of mutants |
|---|---|---|---|
WT | – | – | 0.56 ± 0.08 |
1 | 32.1 | 6.4 | 0.44 ± 0.19 |
2 | 48.8 | 36.6 | 0.69 ± 0.33 |
3 | 45.7 | 32.6 | 0.71 ± 0.35 |
4 | 34.8 | 19.1 | 0.58 ± 0.23 |
5 | 56.8 | 54.6 | 0.81 ± 0.31 |
6 | 62.5 | 50.0 | 0.75 ± 0.28 |
7 | 74.5 | 68.1 | 0.98 ± 0.41 |
8 | 69.6 | 52.2 | 0.83 ± 0.43 |