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Bioresources and Bioprocessing

Table 2 Effects of chemical agents on the recombinant AcCR

From: Enzymatic characterization of a recombinant carbonyl reductase from Acetobacter sp. CCTCC M209061

Reagent

Concentration (mM)

Relative activity (%)

Control

 

100.0 ± 0.8

EDTA

2

95.7 ± 2.3

5

92.4 ± 2.9

SDS

2

68.4 ± 1.2

5

53.4 ± 1.4

β-Mercaptoethanol

20

94.2 ± 2.0

40

88.1 ± 2.0

Iodoacetamide

20

97.1 ± 1.4

40

85.3 ± 1.2

Tween-80

1% (v/v)

74.1 ± 1.9

2% (v/v)

66.9 ± 1.8

Triton X-100

1% (v/v)

86.8 ± 1.9

2% (v/v)

71.0 ± 2.0

  1. Reaction conditions: 0.25 mM NADH, 2 mL 50 mM citrate–phosphate buffer (pH 6.5) with different chemical agents, 5 mM 4′-chloroacetophenone were incubated for 10 min at 35 °C before adding 20 μL purified recombinant AcCR (about 0.008 mg of the purified enzyme), and the changes of absorbance for 3 min were recorded
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