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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Biological conversion of methanol by evolved Escherichia coli carrying a linear methanol assimilation pathway

Fig. 1

Assembly of linear methanol utilization pathway in E. coli. a Schematic illustration of methanol utilization by introducing heterologous pathway in E. coli. MDH methanol dehydrogenase, FLS formolase, DHAK dihydroxyacetone kinase, DHA dihydroxyacetone, DHAP dihydroxyacetone phosphate. The red cross represents native formaldehyde detoxification pathway (formaldehyde to CO2) is blocked by knocking out formaldehyde dehydrogenase gene frmA. b Specific activity of MDH in recombinant E. coli strains. ± indicates standard deviation (n = 3). ND indicates that no MDH activity was detected. c SDS-PAGE analysis of MDH and FLS overexpression. Lane M, marker; lane 1, crude extract of Ec-ΔfrmA-pTrc99A; lane 2, crude extract of Ec-ΔfrmA-mdh3 MGA3-fls; lane 3, Ec-ΔfrmA-mdh2 PB1-fls

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