Table 2 Screening of factors for amylase production from strain ALA4 by two-level factorial design
Run order | pH (A) | Temperature (B) | Moisture (C) | Starch (D) | Yeast extract (E) | Amylase activity (U/g) | |
|---|---|---|---|---|---|---|---|
Experimental | Predicted | ||||||
1 | 8 | 37 | 90 | 1 | 0.1 | 1983.34 | 2039.43 |
2 | 7 | 37 | 90 | 1 | 0.1 | 1723.45 | 1651.02 |
3 | 8 | 37 | 90 | 0.5 | 0.5 | 2012.14 | 2053.55 |
4 | 8 | 42 | 100 | 0.5 | 0.5 | 2534.21 | 2521.03 |
5 | 7 | 37 | 100 | 0.5 | 0.1 | 2412.13 | 2393.05 |
6 | 8 | 42 | 90 | 1 | 0.1 | 2156.14 | 2111.78 |
7 | 7 | 37 | 100 | 1 | 0.5 | 2672.12 | 2653.35 |
8 | 7 | 37 | 90 | 1 | 0.5 | 1512.34 | 1584.46 |
9 | 7 | 42 | 100 | 0.5 | 0.1 | 2112.23 | 2164.67 |
10 | 8 | 37 | 100 | 1 | 0.1 | 2145.12 | 2171.43 |
11 | 7 | 42 | 100 | 1 | 0.5 | 2042.12 | 2126.83 |
12 | 8 | 42 | 100 | 0.5 | 0.1 | 2012.13 | 2013.10 |
13 | 7 | 42 | 100 | 1 | 0.1 | 2214.51 | 2162.07 |
14 | 7 | 37 | 90 | 0.5 | 0.1 | 1967.12 | 2039.55 |
15 | 8 | 42 | 90 | 0.5 | 0.1 | 2214.14 | 2258.50 |
16 | 7 | 42 | 100 | 0.5 | 0.5 | 2224.14 | 2129.43 |
17 | 7 | 37 | 90 | 0.5 | 0.5 | 2045.12 | 1973.00 |
18 | 7 | 42 | 90 | 0.5 | 0.5 | 2136.34 | 2192.04 |
19 | 8 | 42 | 90 | 1 | 0.5 | 2245.12 | 2277.26 |
20 | 8 | 37 | 90 | 0.5 | 0.1 | 1912.23 | 1856.14 |
21 | 7 | 42 | 90 | 1 | 0.5 | 2245.14 | 2189.44 |
22 | 8 | 37 | 90 | 1 | 0.5 | 2278.26 | 2236.85 |
23 | 8 | 42 | 90 | 0.5 | 0.5 | 2456.12 | 2423.98 |
24 | 7 | 42 | 90 | 0.5 | 0.1 | 2267.86 | 2244.44 |
25 | 8 | 37 | 100 | 1 | 0.5 | 2219.56 | 2178.56 |
26 | 7 | 37 | 100 | 0.5 | 0.5 | 3023.12 | 3041.89 |
27 | 7 | 42 | 90 | 1 | 0.1 | 2218.43 | 2241.85 |
28 | 8 | 42 | 100 | 1 | 0.1 | 1867.34 | 1866.37 |
29 | 7 | 37 | 100 | 1 | 0.1 | 1985.43 | 2004.51 |
30 | 8 | 42 | 100 | 1 | 0.5 | 2361.12 | 2374.31 |
31 | 8 | 37 | 100 | 0.5 | 0.5 | 1954.27 | 1995.27 |
32 | 8 | 37 | 100 | 0.5 | 0.1 | 2014.45 | 1988.14 |