Ionic liquid-based enzyme-assisted extraction of chlorogenic acid from Flos Lonicera Japonicae

Bioresources and Bioprocessing

Table 2 The percentage of secondary structure elements and enzyme activity

Sample	α-helix (%)	β-sheet (%)	β-turn (%)	Random coil (%)	Enzyme activity (u/mg)
Original pectinase	11	33	20	36	100^a
Aqueous solution pectinase	9.6	29.2	21	40.2	86.1^b
IL-pectinase	20	19	24	37	123.5^c

^aUntreated
^bTreated under a condition of extraction time 40 min, extraction temperature 70 °C, pH 4.0, 1 mg/ml pectinase, and the solution was aqueous solution
^cTreated under a condition of 0.75 M [C6mim]Br, extraction time 40 min, extraction temperature 70 °C, pH 4.0, 1 mg/ml pectinase, and the solution was IL aqueous solution