Bioresources and Bioprocessing

Table 3 Effect of chemicals on KerP activity

From: Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Chemicals	Concentrations	Relative activity (%)
Control	–	100 ± 2
CaCO₃	10 mM	125 ± 2
MgSO₄	10 mM	151 ± 1
MnSO₄	10 mM	158 ± 2
FeSO₄	10 mM	308 ± 2
ZnSO₄	10 mM	60 ± 1
NiSO₄	10 mM	15 ± 1
EDTA	1 mM	47 ± 1
PMSF	1 mM	8 ± 1
SDS	1%	279 ± 2
β-mercaptoethanol	1%	31 ± 1
Triton X-100	1%	109 ± 5
Tween-80	1%	100 ± 1

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