Skip to main content
Bioresources and Bioprocessing

Table 1 Catalytic parameters of wild-type CueO and mutants

From: Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge

 

K m (μmol/L)

k cat (s−1)

k cat/K m

CueO

292.5 ± 40.0

31.7 ± 0.8

0.108 ± 0.018

G276R

493.3 ± 60.6

60.8 ± 1.8

0.123 ± 0.019

G276K

219.0 ± 40.2

63.1 ± 2.6

0.288 ± 0.065

G276Y

269.4 ± 29.5

38.3 ± 1.1

0.142 ± 0.020

G276N

123.7 ± 16.8

35.0 ± 1.0

0.283 ± 0.046

  1. The kinetic parameters were measured under optimal temperatures and pHs of CueO, G276R, G276K, G276Y, and G276N (70, 60, 30, 50, and 50 °C and 3.5, 3.5, 2.5, 2.5, and 2.5, respectively). The experiments were performed using ABTS (0.2–10 mM), 8 mM Cu2+, and citrate phosphate buffer. Measurements were performed using standard enzyme assays in three replications. The data were fit using GraphPad Prism 5.0 (GraphPad Software Inc.)
Back to article page