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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

Fig. 3

Number of amino acid substitutions (always the first column) which lead to improved (+), unchanged (=), decreased (−) organic cosolvent resistance and inactive variants (×) in a DMSO, b DOx, and c TFE. d Distribution of the 20 amino acids according to the genetic code (right) and amino acids composition of WT BSLA (left). Grouped into aromatic (F, Y, and W), aliphatic (A, V, L, I and G), charged (D, E, H, K and R), and polar (C, M, P, S, T, N and Q). For each organic solvent resistance group (+, =, −, ×), the left bar displays the total number of variants, and the right shows the distribution of the amino acids. +: variants with improved resistance; =: unchanged resistance; −: decreased resistance; ×: inactive in buffer

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