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Bioresources and Bioprocessing

Table 1 Screening system of BSLA in three organic cosolvents

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

Organic cosolvent

Conc. (m)a

RWT (%)

Standard deviation (%)

(% v/v)

Apparent

True

DMSO

8.4 (≈ 60.0)

29.0

8.0

10.5

DOx

2.6 (≈ 22.0)

31.0

6.5

9.6

TFE

2.9 (≈ 12.0)

30.0

7.6

12.0

  1. Conc. concentration, R WT residual activity of BSLA WT. Apparent and true standard deviation values for BSLA WT in the respective organic cosolvents, DMSO dimethyl sulfoxide, DOx 1,4-dioxane, TFE 2,2,2-trifluoroethanol
  2. aConcentration used in the incubation step for the BSLA activity assay
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