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Table 1 Screening system of BSLA in three organic cosolvents

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

Organic cosolvent Conc. (m)a RWT (%) Standard deviation (%)
(% v/v) Apparent True
DMSO 8.4 (≈ 60.0) 29.0 8.0 10.5
DOx 2.6 (≈ 22.0) 31.0 6.5 9.6
TFE 2.9 (≈ 12.0) 30.0 7.6 12.0
  1. Conc. concentration, R WT residual activity of BSLA WT. Apparent and true standard deviation values for BSLA WT in the respective organic cosolvents, DMSO dimethyl sulfoxide, DOx 1,4-dioxane, TFE 2,2,2-trifluoroethanol
  2. aConcentration used in the incubation step for the BSLA activity assay