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Table 2 Effect of each type of amino acid substitution on organic cosolvent resistance of BSLA

From: Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

  AA substitutions from each type of AA in BSLA WT
15 Aromatic AA in BSLA WT (300 substitutions) 79 Aliphatic AA in BSLA WT (1580 substitutions) 54 polar AA in BSLA WT (1080 substitutions) 33 charged AA in BSLA WT (660 substitutions)
  + (44) = (193) − (36) × (27) + (155) = (843) − (269) × (313) + (108) = (759) − (136) × (77) + (64) = (417) − (98) × (81)
DMSO
 Aromatic 7 20 8 0 9 14 17 19 8 14 24 19 22 12 21 15
 Aliphatic 32 26 17 19 21 32 20 12 23 28 16 16 25 24 30 25
 Polar 27 36 33 44 41 36 39 27 32 36 34 29 25 35 36 43
 Charged 34 18 42 37 29 18 24 42 36 22 26 36 28 29 13 17
  + (19) = (195) − (59) × (27) + (60) = (827) − (380) × (313) + (56) = (780) − (167) × (77) + (24) = (486) − (69) × (81)
DOx
 Aromatic 5 22 3 0 17 13 15 19 16 13 22 19 17 14 19 15
 Aliphatic 26 26 25 19 13 34 18 12 16 28 19 16 17 26 22 25
 Polar 21 35 34 44 32 37 39 27 32 37 32 29 25 35 32 43
 Charged 47 17 37 37 38 16 28 42 36 23 27 36 42 25 28 17
  + (12) = (211) − (50) × (27) + (69) = (836) − (362) × (313) + (62) = (751) − (190) × (77) + (38) = (479) − (62) × (81)
TFE
 Aromatic 8 19 6 0 23 12 17 19 18 15 14 19 16 15 16 15
 Aliphatic 0 27 28 19 14 33 20 12 19 26 28 16 16 26 23 25
 Polar 33 34 34 44 29 38 37 27 26 38 31 29 45 33 32 43
 Charged 58 20 32 37 33 18 26 42 37 22 28 36 24 26 29 17
  1. Twelve patterns per organic solvent are shown. The absolute number of AA substitutions per type is shown in brackets
  2. Amino acid groups: aromatic (F, Y, and W), aliphatic (A, V, L, I, and G), charged (D, E, H, K, and R), and polar (C, M, P, S, T, N, and Q). For activity group definitions (+, =, −, ×), see text. Values are given as percentages for easier comparison, as BSLA amino acid composition is not evenly distributed (43.6% aliphatic, 29.8% polar, 18.2% charged and 8.3% aromatic AA)